Protein_Domain

Part:BBa_K4803000:Design

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)


gp130 (IL 6 receptor-beta) extracellular domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 9
    Illegal BamHI site found at 827
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We performed codon optimization to ensure effective expression of this part in human cells.

We also used simulations to predict and confirm that the steric structure is not disrupted even when the amino acid sequence is only in the extracellular domain.

Major restriction enzyme recognition sequences (EcoRI XbaI SpeI PstI NotI BsaⅠ SapⅠ) are removed from this part.

Source

The sequence of this part is an extracellular domain generated by codon optimization of the gp130 cDNA described in [1], minus the 5' end signal and downstream of the transmembrane region.

Reference

[1] Hibi, M., Murakami, M., Saito, M., Hirano, T., Taga, T., & Kishimoto, T. (1990). Molecular cloning and expression of an IL-6 signal transducer, gp130. Cell, 63(6), 1149-1157. https://doi.org/10.1016/0092-8674(90)90411-7